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Buy Wholesale and maintain an Active status for 2 months and we will refund your $39 Distributor Fee
09-SEPTEMBER-2008 19:33:10 - HL60 Redirected from HL-60 The HL-60 Human promyelocytic leukemia cells cell line is a leukemic cell line that has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient medium supplemented with fetal bovine serum, L-glutamine, HEPES and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at the National Cancer Institute.1 HL-60 cells are predominantly a neutrophilic promyelocyte precursor.1 Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.2 With this line, spontaneous differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide DMSO, or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate TPA and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively. The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. HL-60 cell model was used to study the effect of DNA topoisomerase topo IIα and IIβ on differentiation and apoptosis of cells3 and is especially useful in dielectrophoresis studies,4 which require an aqueous environment with suspended and round cells. References ^ a b Gallagher R, Collins S, Trujillo J, et al 1979. Characterization of the continuous, differentiating myeloid cell line HL-60 from a patient with acute promyelocytic leukemia. Blood 54 3: 713-33. PMID 288488. ^ Breitman, T, S. Collins, B. Keene, 1980. Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60. Exp. Cell Res., 126, 494-498. ^ Sugimoto, K, K. Yamada, M. Egashira, Y. yazaki, H. Hirai, A. Kikuchi and K. Oshimi, 1998. Temporal and Spatial Distribution of DNA Topoisomerase II Alters During Proliferation, Differentiation, and Apoptosis in HL-60 Cells. Blood, 91:4, 1407-1417. ^ Ratanachoo, K., Gascoyne, P.R.C. and Ruchirawat, M. 2002. Detection of cellular responses to toxicants by dielectrophoresis. BBA. 1564, 449-458 Retrieved from http://en..org/wiki/HL60 Categories: Cell lines Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page This page was last modified on 13 March 2008, at 09:5
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So many of us do not eat a balanced diet, get enough sleep, have too much stress, or are impacted with toxins and pollutants. Drinking 2 ounces of MonaVie twice a day will help your body detoxify as well as build your immune system. Its the smartest thing you can do for yourself, so start today. Buying MonaVie through our company guarantees you support 7 days a week and, if you would like to share MonaVie with your family and friends we will guide you from start to finish.
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