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News About Folin_Ciocalteu_reagent

22-AUGUST-2008 06:13:22 - Folin-Ciocalteu reagent The Folin-Ciocalteu reagent or Folin's phenol reagent or Folin-Denis reagent is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic antioxidants and polyphenol antioxidants.1 It works by measuring the amount of the substance being tested needed to inhibit the oxidation of the reagent.2 However, this reagent does not only measure total phenols and will react with any reducing substance. The reagent therefore measures the total reducing capacity of a sample, not just the level of phenolic compounds. This reagent forms part of the Lowry protein assay and will also react with some nitrogen-containing compounds such as hydroxylamine and guanidine.3 This reagent should not be confused with Folin's reagent, which is used to detect amines and sulfur-containing compounds. References ^ Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 1999; 299: 152-178. ^ Vinson J, Zubik L, Bose P, Samman N, Proch J 2005. Dried fruits: excellent in vitro and in vivo antioxidants. J Am Coll Nutr 24 1: 44-50. PMID 15670984. ^ Ikawa M, Schaper TD, Dollard CA, Sasner JJ 2003. Utilization of Folin-Ciocalteu phenol reagent for the detection of certain nitrogen compounds. J. Agric. Food Chem. 51 7: 1811-5. doi:10.1021/jf021099r. PMID 12643635. External links Folin-Ciocalteu's phenol reagent Fluka data sheet. This biochemistry article is a stub. Retrieved from http://en..org/wiki/Folin-Ciocalteu_reagent Categories: Biochemistry stubs | Chemical tests Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page This page was last modified on 7 June 2008, at 16:06. of the GNU Free Documentation License. ® , Inc., a U.S.

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