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20-September-2008 09:55:54 - Glycosyltransferase Peptidoglycan biosynthesis glycosyltransferase MurG 1f0k. Blue plane shows hydrocarbon boundary of the lipid bilayer Peptidoglycan biosynthesis glycosyltransferase MurG 1f0k. Blue plane shows hydrocarbon boundary of the lipid bilayer Glycosyltransferases are enzymes EC 2.4 that act as a catalyst for the transfer of a monosaccharide unit from an activated sugar phosphate known as the glycosyl donor to an acceptor molecule, usually an alcohol. The result of glycosyl transfer can be a monosaccharide glycoside, an oligosaccharide, or a polysaccharide, although some glycosyltransferases catalyse transfer to inorganic phosphate or water. Glycosyl transfer can also occur to protein residues, usually to tyrosine, serine or threonine to give O-linked glycoproteins, or to asparagine to give N-linked glycoproteins. Mannosyl groups may be transferred to tryptophan to generate C-mannosyl tryptophan, which is relatively abundant in eukaryotes. Commonly, sugar nucleotide derivatives are used as glycosyl donors. Glycosyltransferases that use sugar nucleotides are called Leloir enzymes, after Luis F. Leloir, the scientist who discovered the first sugar nucleotide and who received the 1970 Nobel Prize in Chemistry for his work on carbohydrate metabolism. Glycosyltransferases that utilize non-nucleotide donors, which may be polyprenol pyrophosphates, polyprenol phosphates, sugar-1-phosphates or sugar-1-pyrophosphates, are termed non-Leloir glycosyltransferases. Such non-Leloir enzymes occur in a variety of organisms. Contents 1 Mechanism 2 Classification by sequence 3 Structure 4 Inhibitors 5 Uses 6 See also 7 References 8 External links Mechanism Glycosyltransferases, by analogy with glycoside hydrolases, can catalyze the transfer of a glycosyl moiety with either retention or inversion of configuration. Glycosyltransferases are usually metal ion dependent with metals such as magnesium or manganese being found in the active site and acting as a Lewis acid by binding to the diphosphate leaving group. Mammals utilize only 9 sugar nucleotide donors for glycosyltransferases: UDP-glucose, UDP-galactose, UDP-GlcNAc, UDP-GalNAc, UDP-xylose, UDP-glucuronic acid, GDP-mannose, GDP-fucose, and CMP-sialic acid. Other organisms have an extensive range of sugar nucleotide donors. Many glycosyltransferases in a number of organisms use lipid linked glycosyl donors where the lipid is frequently a terpenoid such as dolichol or polyprenol. Classification by sequence Sequence based classification methods have proven to be a powerful way of generating hypotheses for protein function based on sequence alignment to related proteins. The carbohydrate active enzyme database presents a sequence based classification of glycosyltransferases into over 86 families. The same three-dimensional fold is expected to occur within each of the families.1 Structure In contrast to the diversity of 3D structures observed for glycoside hydrolases, glycosyltransferase have a much smaller range of structures. In fact, according to the Structural Classification of Proteins database only three different folds have been observed for glycosyltransferases2 Very recently, a new glycosyltransferase fold was identified for the glycosyltransferases involved in the biosynthesis of the NAG-NAM polymer backbone of peptidoglycan.3 Inhibitors Many inhibitors of glycosyltransferases are known. Some of these are natural products, such as moenomycin, an inhibitor of peptidoglycan glycosyltransferases, the nikkomycins, inhibitors of chitin synthase, and the echinocandins, inhibitors of fungal b-1,3-glucan synthases. Some glycosyltransferase inhibitors are of use as drugs or antibiotics. Moenimycin is used in animal feed as a growth promoter. Caspofungin has been developed from the echinocandins and is in use as an antifungal agent. Ethambutol is an inhibitor of mycobacterial arabinotransferases and is used for the treatment of tuberculosis. Lufenuron is an inhibitor of insect chitin synthases and is used to control fleas in animals. Uses Glycosyltransferases have been widely used in the synthesis of glycoconjugates. Suitable enzymes can be isolated from natural sources or produced recombinantly. Alternatively, whole cell based systems utilizing either endogenous glycosyl donors or cell based systems containing cloned and expressed systems for synthesis of glycosyl donors have been developed. In cell-free approaches the large scale application of glycosyltransferases for glycoconjugate synthesis has required access to large quantities of the glycosyl donors. Alternatively, nucleotide recycling systems have been developed that allow the resynthesis of glycosyl donors from the released nucleotide. The nucleotide recycling approach has a further benefit of reducing the amount of nucleotide formed as a by-product, thereby reducing the amount of inhibition caused to the glycosyltransferase of interest - a commonly observed feature of the nucleotide byproduct. See also Oligosaccharyltransferase Glucuronosyltransferase Glycogen synthase Glycoside hydrolase References ^ http://cazy.org ^ SCOP: Structural Classification of Proteins ^ Lovering A, de Castro L, Lim D, Strynadka N 2007. Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis. Science 315 5817: 1402-5. doi:10.1126/science.1136611. PMID 17347437. External links UMich Orientation of Proteins in Membranes families/superfamily-136 - Orientation of Peptidoglycan biosynthesis glycosyltransferase MurG in membrane v d e Transferases: glycosyltransferases EC 2.4 2.4.1 - Hexosyltransferases Glucosyltransferase Phosphorylase Starch, Glycogen, Myo- - Glycogen synthase - Debranching enzyme - Branching enzyme - 1,3-beta-glucan synthase - Ceramide glucosyltransferase Galactosyltransferase Lactose synthase - B-N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase Glucuronosyltransferase UGT1A1 - UGT2B7 - Hyaluronan synthase Fucosyltransferase POFUT1 - POFUT2 2.4.2 - Pentosyltransferases ADP ribose Cholera toxin - Diphtheria toxin - Pertussis toxin - Poly ADP ribose polymerase Other Purine nucleoside phosphorylase - Adenine phosphoribosyltransferase - Hypoxanthine-guanine phosphoribosyltransferase - Uracil phosphoribosyltransferase - Amidophosphoribosyltransferase - Xylosyltransferase Retrieved from http://en..org/wiki/Glycosyltransferase Categories: Transferases | EC 2.4 | EC 2.4.1 | EC 2.4.2 | Peripheral membrane proteins Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page Languages Deutsch This page was last modified on 28 May 2008, at 21:01
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