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14-September-2008 12:50:20 - Anatomical pathology Autopsy: a brain surrounded by pus the yellow-greyish coat around the brain, under the dura lifted by the forceps, the result of bacterial meningitis. Autopsy: a brain surrounded by pus the yellow-greyish coat around the brain, under the dura lifted by the forceps, the result of bacterial meningitis. Gross examination: appearance of the cut surface of a lung showing the honeycomb pattern of end-stage pulmonary fibrosis. Gross examination: appearance of the cut surface of a lung showing the honeycomb pattern of end-stage pulmonary fibrosis. Gross examination: appearance of a colorectal polyp the cauliflower-shaped tumor attached to the colon mucosa the horizontal line at the bottom. Gross examination: appearance of a colorectal polyp the cauliflower-shaped tumor attached to the colon mucosa the horizontal line at the bottom. Histopathology: microscopic appearance of invasive ductal carcinoma of the breast. The slide is stained with Haematoxylin Eosin. Histopathology: microscopic appearance of invasive ductal carcinoma of the breast. The slide is stained with Haematoxylin Eosin. Histopathology: microscopic appearance of invasive ductal carcinoma of the breast. The slide is stained with an antibody immunohistochemistry against the ongene Her2neu. The dark brown reaction indicates that this tumor over-expresses this gene. Histopathology: microscopic appearance of invasive ductal carcinoma of the breast. The slide is stained with an antibody immunohistochemistry against the ongene Her2neu. The dark brown reaction indicates that this tumor over-expresses this gene. Cytopathology: microscopic appearance of a Pap test. The pink cell a the center with a large nucleus is abnormal, compatible with low grade dysplasia. Cytopathology: microscopic appearance of a Pap test. The pink cell a the center with a large nucleus is abnormal, compatible with low grade dysplasia. Anatomical pathology Commonwealth or Anatomic pathology U.S. is a medical specialty that is concerned with the diagnosis of disease based on the gross, microscopic, and molecular examination of organs, tissues, and whole bodies autopsy. The anatomical pathologist reports to doctors; he/she does not usually see patients. Anatomical pathology is itself divided in subspecialties, the main ones being surgical pathology, cytopathology and forensic pathology. To be licensed to practice anatomical pathology, one has to complete medical school, an approved residency program and be certified. In the U.S., the American board of Pathology certifies pathologists. Anatomical pathology is one of two branches of pathology, the other being clinical pathology, the diagnosis of disease through the laboratory analysis of bodily fluids. Often, pathologists practice both anatomical and clinical pathology, a combination known as general pathology. Similar specialties exist in veterinary pathology. Contents 1 Skills and procedures 2 Subspecialties 2.1 Surgical pathology 2.2 Oral and maxillofacial pathology 2.3 Cytopathology 2.4 Molecular pathology 2.5 Autopsy pathology 2.6 Forensic pathology 3 Training and certification of Anatomical Pathologists 3.1 Australia 3.2 Canada 3.3 USA 4 Anatomical pathology practice settings 5 See also 6 External links 7 References Skills and procedures The procedures used in anatomic pathology include: Gross examination - the examination of diseased tissues with the naked eye. This is important especially for large tissue fragments, because the disease can often be visually identified. It is also at this step that the pathologist selects areas that will be processed for histopathology. The eye can sometimes be aided with a magnifying glass or a stereo microscope, especially when examining parasitic organisms. Histopathology - the microscopic examination of stained tissue sections using histological techniques. The standard stains are haematoxylin and eosin, but many others exist. The use of haematoxylin and eosin-stained slides to provide specific diagnoses based on morphology is considered to be the core skill of anatomic pathology. The science of staining tissues sections is called histochemistry. Immunohistochemistry - the use of antibodies to detect the presence, abundance, and localization of specific proteins. This technique is critical to distinguishing between disorders with similar morphology, as well as characterizing the molecular properties of certain cancers. In situ hybridization - Specific DNA and RNA molecules can be identified on sections using this technique. When the probe is labeled with fluorescent dye, the technique is called FISH. Cytopathology - the examination of loose cells spread and stained on glass slides using cytology techniques. Electron microscopy - the examination of tissue with an electron microscope, which allows much greater magnification, enabling the visualization of organelles within the cells. Its use has been largely supplanted by immunohistochemistry, but it is still in common use for certain tasks, including the diagnosis of kidney disease and the identification of immotile cilia syndrome among many others. Tissue cytogenetics - the visualization of chromosomes to identify genetics defects such as chromosomal translocation. Flow immunophenotyping - the determination of the immunophenotype of cells using flow cytometry techniques. It is very useful to diagnose the different types of leukemia and lymphoma. Subspecialties Surgical pathology Main article: Surgical pathology Surgical pathology is the most significant and time-consuming area of practice for most anatomical pathologists. Surgical pathology involves the gross and microscopic examination of surgical specimens, as well as biopsies submitted by non-surgeons such as general internists, medical subspecialists, dermatologists, and interventional radiologists. Oral and maxillofacial pathology In the United States, subspecialty-trained doctors of dentistry, rather than medical doctors, can be certified by a professional board to practice Oral and Maxillofacial Pathology. Cytopathology Main article: Cytopathology Cytopathology is a sub-discipline of anatomical pathology concerned with the microscopic examination of whole, individual cells obtained from smears or fine needle aspirates. Cytopathologists are trained to perform fine-needle aspirates of superficially located organs, masses, or cysts, and are often able to render an immediate diagnosis in the presence of the patient and consulting physician. In the case of screening tests such as the Papanicolaou smear, non-physician cytotechnologists are often employed to perform initial reviews, with only positive or uncertain cases examined by the pathologist. Cytopathology is a board-certifiable subspecialty in the U.S. Molecular pathology Main article: Molecular pathology Molecular pathology is an emerging discipline within anatomical pathology which is focused on the use of nucleic acid-based techniques such as in-situ hybridization, reverse-transcriptase polymerase chain reaction, and nucleic acid microarrays for specialized studies of disease in tissues and cells. Molecular pathology shares some aspects of practice with both anatomic and clinical pathology, and is sometimes considered a crossover discipline. Autopsy pathology Main article: Autopsy General anatomical pathologists are trained in performing autopsies, which are used to determine the disease factors contributing to a person's death. Autopsies are important in the ongoing medical education of clinicians, and in efforts to improve and verify the quality of medical care. Dieners are non-physicians who assist pathologists in the gross dissection portion of the autopsy. The recently established field of Pathologists Assistant has taken over a the role of Diener and contributes a great deal to the pre-diagnosis of disease within the pathology laboratory. Autopsies represent less than 10% of the workload of typical pathologists in the United States.1 However, the autopsy is central to public perceptions of the field, in part due to portrayals of pathologists on television programs such as Quincy, M.E. and Silent Witness. Forensic pathology Main article: Forensic pathology Forensic pathologists receive specialized training in determining the cause of death and other legally relevant information from the bodies of persons who died in a non-medical or potentially criminal circumstances. Autopsies make up much, but not all of the work of the practicing forensic pathologist, and forensic pathologists are occasionally consulted to examine a survivor of a criminal attack. Forensic pathology is a board-certifiable sub-specialty in the U.S. Training and certification of Anatomical Pathologists Australia Also New Zealand, Hong Kong, Singapore and Malaysia Anatomical Pathology one of the specialty training programs offered by the Royal College of Pathologists of Australasia RCPA. The RCPA article is here. To qualify as a Fellow of the RCPA in Anatomical Pathology, the candidate must complete a recognised undergraduate or postgraduate medical qualification, then complete 2 years of clinical medical experience Internship and a general clinical residency year as a prerequisite to selection as a training registrar. The training program is a minimum of 5 years, served in at least two laboratories, and candidates must pass a Basic Pathological Sciences examination usually in first year, the Part 1 examination not before 3rd year and the Part 2 examination not before 5th year. Fellows may then continue into subspecialty training. Canada Anatomical Pathology AP is one of the specialist certificates granted by the Royal College of Physicians and Surgeons of Canada. Other certificates related to pathology include general pathology GP, forensic pathology, hematopathology, and neuropathology. Candidates for any of these must have completed four years of medical school and five years of residency training. After becoming certified in either AP or GP, it is common for pathologists to seek further fellowship training in a subspecialty of AP. USA Anatomic Pathology AP is one of the two primary certifications offered by the American Board of Pathology. The other is Clinical Pathology CP. To be certified in anatomic pathology, the trainee must complete four years of medical school followed by three years of residency training. Many US pathologists are certified in both AP and CP, which requires a total of four years of residency. After completing residency, many pathologists enroll in further years of fellowship training to gain expertise in a subspecialty of AP. Anatomical pathology practice settings Academic anatomical pathology is practiced at university medical centers by pathologists who are also university faculty. As such, they often have diverse responsibilities that may include training pathology residents, teaching medical students, conducting basic, clinical, or translational research, and/or performing administrative duties, all in addition to the practice of diagnostic anatomical pathology. Pathologists in academic settings often sub-specialize in a particular area of anatomic pathology and may serve as consultants to other pathologists regarding cases in their specific area of expertise. Group practice is the most traditional private practice model. In this arrangement, a group of senior pathologists will control a partnership that employs junior pathologists and contracts independently with hospitals to provide diagnostic services, as well as attracting referral business from local clinicians who practice in the outpatient setting. The group often owns a laboratory for histology and ancillary testing of tissue, and may hold contracts to run hospital-owned labs. Many pathologists who practice in this setting are trained and certified in both anatomical pathology and clinical pathology, which allows them to supervise blood banks, clinical chemistry laboratories, and medical microbiology laboratories as well. Large corporate providers of anatomical pathology services have emerged in recent years, most notably AmeriPath in the United States. In this model, pathologists are employees, rather than independent partners. This model has been criticized for reducing physician independence, but defenders claim that the larger size of these practices allow for economies of scale and greater specialization, as well a sufficient volume to support more specialized testing methods. Multispecialty groups, composed of physicians from clinical specialties as well as radiology and pathology, are another practice model. In some case, these may be large groups controlled by an HMO or other large health care organization. In others, they are essentially clinician group practices that employ pathologists to provide diagnostic services for the group. These groups may own their own laboratories, or, in some cases may make controversial arrangements with pod labs that allow clinician groups to lease space, with the clinican groups receiving direct insurance payments for pathology services.2 Proposed changes to Medicare regulations may essentially eliminate these arrangements in the United States.3 See also Pathology Clinical pathology Forensic pathology Veterinary pathology Plant pathology Histology Laser capture microdissection External links PathMax, a collection of online Pathology resources MyBiopsy.org, information on more than 25 of the most common cancers and cancer-related conditions The Doctor's doctor, a very useful web site for patients and pathologists Pathologie Online, online Pathology resources in German Pathology Outlines, an online textbook of anatomic pathology College of American Pathologists American Board of Pathology CYTOPATHNET Online Resource Center for Cytopathology Histology Group of Victoria Incorporated Flickr group: Pathology and Lab Medicine: numerous photos illustrating the work of pathologists. PathologyPics.com: An interactive histology database for the Practicing Anatomic Pathologist as well as Pathology Trainees. References ^ Smith R D 1989. Some characteristics of the community practice of pathology in the United States. National Manpower Survey of 1987.. Arch Pathol Lab Med 113 12: 1335-42. PMID 2589945. ^ 1 Congress of American Pathologists Feature story - Out of joint OIG takes dim view of pod lab setup January, 2005 Accessed 5-19-2007 ^ 2 Foster, Swift, Collins, and Smith, P.C. - Health Care Alert, August 2006. v d e Pathology Principles of pathology Disease - Infection - Ischemia - Inflammation - Wound healing - Neoplasia - Hemodynamics Cell death: Necrosis Liquefactive necrosis, Coagulative necrosis, Caseous necrosis - Apoptosis - Pyknosis - Karyorrhexis - Karyolysis Cellular adaptation: Atrophy - Hypertrophy - Hyperplasia - Dysplasia - Metaplasia Squamous, Glandular accumulations: pigment Hemosiderin, Lipochrome/Lipofuscin, Melanin - Steatosis Anatomical pathology Surgical pathology - Cytopathology - Autopsy - Molecular pathology - Forensic pathology - Dental pathology Gross examination - Histopathology - Immunohistochemistry - Electron microscopy - Immunofluorescence - Fluorescent in situ hybridization Clinical pathology Clinical chemistry - Hematopathology - Transfusion medicine - Medical microbiology - Diagnostic immunology - Immunopathology Enzyme assay - Mass spectrometry - Chromatography - Flow cytometry - Blood bank - Microbiological culture - Serology Retrieved from http://en..org/wiki/Anatomical_pathology Categories: Anatomical pathology Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page Languages Asturianu Català Dansk Español Euskara Français Bahasa Indonesia Italiano Nederlands Polski Português Română SlovenÄ?ina ไทย This page was last modified on 6 September 2008, at 04:16
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