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Buy Wholesale and maintain an Active status for 2 months and we will refund your $39 Distributor Fee
14-September-2008 18:38:50 - AP site An AP site meaning apurinic and apyrimidinic is a location in DNA that have neither a purine nor pyrimidine base, usually due to DNA damage. If left unrepaired AP sites can lead to mutation during semiconservative replication as a random nucleotide base will be inserted into the strand synthesised opposite them. AP sites are repaired by AP endonucleases. These remove a stretch of one or more nucleotides on the 5' side of the AP site, then fresh nucleotide bases are inserted using the intact DNA strand as a template. If only one nucleotide is being repaired, then the so-called short patch repair mechanism is employed where DNA Ligase II, DNA Polβ and XRCC1 are used. If more than one nuceotide is being replaced, then long patch machinery is employed in which DNA Polδ or Polε as well as PCNA, Fen-1 and DNA ligase I bridge the gap. Some of these factors are also involved in DNA replication such as PCNA. References Griffiths, Anthony J. et al 2005. Introduction to Genetic Analysis 8th Ed.. W.H. Freeman. ISBN 0-7167-4939-4 Huberman, Joel A. 2005. The web site for the DNA Repair lectures in RPN530, Oncology for Scientists, at Roswell Park Cancer Institute This biochemistry article is a stub. Retrieved from http://en..org/wiki/AP_site Categories: Molecular genetics | DNA repair | Biochemistry stubs Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page Languages Polski This page was last modified on 15 February 2008, at 17:4
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