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14-September-2008 18:38:38 - 3-Amino-1,2,4-triazole Redirected from Aminotriazole 3-Amino-1,2,4-triazole1 IUPAC name 3-Amino-1,2,4-triazole Other names 1,2,4-Triazol-3-amine aminotriazole Amitrol Amitrole Identifiers Abbreviations 3-AT CAS number 61-82-5 EINECS number 200-521-5 RTECS number XZ3850000 SMILES NC1=NNC=N1 Properties Molecular formula C2H4N4 Molar mass 84.08 Density 1.138 g/mol Melting point 157 - 159 °C Solubility in water 280 gm/liter Hazards R-phrases R48/22 R51/53 R63 S-phrases S2 S13 S36/37 S61 Flash point Nonflammable Except where noted otherwise, data are given for materials in their standard state at 25 °C, 100 kPa Infobox references 3-Amino-1,2,4-triazole 3-AT is a heterocyclic organic compound that consists of a 1,2,4-triazole substituted with an amino group. 3-AT is a competitive inhibitor of the product of the HIS3 gene, imidazoleglycerol-phosphate dehydratase.2 Imidazoleglycerol-phosphate dehydratase is an enzyme catalysing the sixth step of histidine production.3 3-AT is also is a nonselective systemic triazole herbicide used on nonfood croplands to control annual grasses and broadleaf and aquatic weeds. It is not used on food crops because of its carcinogenic properties. As an herbicide, it is known as aminotriazole, amitrole or amitrol. Contents 1 Exploits 2 1959 cranberry contamination 3 References 4 External links Exploits By applying 3-AT to a bacterial cell culture which is dependent upon a plasmid containing HIS3 to produce histidine i.e. its own HIS3 analogue is not present or nonfunctional, an increased level of HIS3 expression is required in order for the bacterial cell to survive. This has proved useful in increasing the affinity of DNA-binding domains selected in one bacterial two-hybrid technique described by Joung et al., in 2000. Since the DNA-binding domain in turn recruits RNA polymerase to the HIS3 gene, a high affinity binding will allow the bacterial cell to survive in media containing higher concentrations of 3-AT. Of course this selection process is performed using selective media, containing no histidine. 1959 cranberry contamination On November 9, 1959, the secretary of the United States Department of Health, Education, and Welfare Arthur S. Flemming announced that some of the 1959 crop was tainted with traces of the herbicide aminotriazole 4. The market for cranberries collapsed and growers lost millions of dollars. References ^ EXTOXNET - Herbicide fact sheet for amitrole ^ Joung, J. K., E. I. Ramm and C. O. Pabo 2000. A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 9713: 7382-7. ^ Yeastgenome.org ^ New York Times website - Opinion section External links Retrieved from http://en..org/wiki/3-Amino-1,2,4-triazole Categories: Genes | Herbicides | Triazoles Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page Languages Deutsch Français Nederlands This page was last modified on 3 June 2008, at 09:42
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