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News About DyLight_Fluor

14-September-2008 18:38:49 - DyLight Fluor Color mass g/mol Absorb nm Emit nm ε M-1cm-1 DyLight 405 violet 793 400 420 30,000 DyLight 488 green 1011 493 518 70,000 DyLight 549 yellow 982 550 568 150,000 DyLight 594 orange 1078 593 618 80,000 DyLight 633 red 1066 638 658 170,000 DyLight 649 red 1008 646 674 250,000 DyLight 680 far-red 950 682 715 140,000 DyLight 750 near-IR 1034 752 778 210,000 DyLight 800 near-IR 1050 770 794 270,000 The DyLight Fluor family of fluorescent dyes is produced by Thermo Fisher Scientific. Conjugated DyLight Dye antibodies are produced by Rockland Immunochemicals, Inc. DyLight Dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology. The excitation and emission spectra of the DyLight Fluor series covers much of the visible spectrum and extends into the infrared region, allowing detection using most fluorescence microscopes, as well as infrared imaging systems. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a green 526 nm laser for DyLight 488, a green 532 nm laser for DyLight 549 and 594, and a red 633 nm laser for DyLight 633 and 649. The DyLight 680, 750 and 800 fluors are compatible with laser-based and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively. DyLight Fluors are synthesized through sulfonate addition to coumarin, xanthene such as fluorescein and rhodamine, and cyanine dyes. Sulfonation makes DyLight Dyes negatively charged and hydrophilic. DyLight Fluors are commercially available as reactive succinimidyl-esters for labeling proteins through lysine residues, and as maleimide derivatives for labeling proteins through cysteine residues. Historically, fluors such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have major limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly. DyLight Fluors have comparable excitation and emission spectra and are generally more photostable, brighter, and less pH-sensitive. References Retrieved from http://en..org/wiki/DyLight_Fluor Categories: Dyes | Fluorescent dyes Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page This page was last modified on 24 June 2008, at 18:21

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Why Drink MonaVie?

So many of us do not eat a balanced diet, get enough sleep, have too much stress, or are impacted with toxins and pollutants. Drinking 2 ounces of MonaVie twice a day will help your body detoxify as well as build your immune system. Its the smartest thing you can do for yourself, so start today. Buying MonaVie through our company guarantees you support 7 days a week and, if you would like to share MonaVie with your family and friends we will guide you from start to finish.

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