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20-September-2008 09:29:09 - hairpin RNA A small hairpin RNA or short hairpin RNA shRNA is a sequence of RNA that makes a tight hairpin turn that can be used to silence gene expression via RNA interference. shRNA uses a vector introduced into cells and utilizes the U6 promoter to ensure that the shRNA is always expressed. This vector is usually passed on to daughter cells, allowing the gene silencing to be inherited. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex RISC. This complex binds to and cleaves mRNAs which match the siRNA that is bound to it. shRNA is transcribed by RNA polymerase III. shRNA production in a mammalian cell can sometimes cause the cell to mount an interferon response as the cell seeks to defend itself from what it perceives as viral attack. This problem is not observed in miRNA, which is transcribed by RNA polymerase II the same polymerase used to transcribe mRNA. shRNAs can also be made for use in plants and other systems, and are not necessarily driven by a U6 promoter. In plants the traditional promoter for strong consitutive expression in most plant species is the cauliflower mosaic virus 35S promoter CaMV35S, in which case RNA Polymerase II is used to express the transcript destined to initiate RNAi. References McIntyre G, Fanning G 2006. Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnol. 6: 1. doi:10.1186/1472-6750-6-1. PMID 16396676. Harper S, Staber P, He X, Eliason S, Martins I, Mao Q, Yang L, Kotin R, Paulson H, Davidson B 2005. RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model. Proc. Natl. Acad. Sci. U.S.A. 102 16: 5820-5. doi:10.1073/pnas.0501507102. PMID 15811941. Nielsen M, Pedersen F, Kjems J 2005. Molecular strategies to inhibit HIV-1 replication. Retrovirology 2: 10. doi:10.1186/1742-4690-2-10. PMID 15715913. Paddison P, Caudy A, Bernstein E, Hannon G, Conklin D 2002. Short hairpin RNAs shRNAs induce sequence-specific silencing in mammalian cells. Genes Dev. 16 8: 948-58. doi:10.1101/gad.981002. PMID 11959843. Cao W, Hunter R, Strnatka D, McQueen C, Erickson R 2005. DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response. J. Appl. Genet. 46 2: 217-25. PMID 15876690. v d e Types of nucleic acids Constituents Nucleobases | Nucleosides | Nucleotides | Deoxynucleotides Ribonucleic acids RNA | mRNA pre-mRNA/hnRNA | tRNA | rRNA | aRNA | gRNA | miRNA | ncRNA | piRNA | shRNA | siRNA | snRNA | snoRNA | stRNA | ta-siRNA | tmRNA Deoxyribonucleic acids DNA | cDNA | gDNA | msDNA | mtDNA Nucleic acid analogues GNA | LNA | PNA | TNA | morpholino Cloning vectors phagemid | plasmid | lambda phage | cosmid | P1 phage | fosmid | BAC | YAC | HAC Major families of biochemicals Saccharides | Carbohydrates | Glycosides | | Amino acids | Peptides | Proteins | Glycoproteins | | Lipids | Terpenes | Steroids | Carotenoids Alkaloids | Nucleobases | Nucleic acids | | Enzyme cofactors | Flavonoids | Polyketides | Tetrapyrroles This biotechnology article is a stub. Retrieved from http://en..org/wiki/Small_hairpin_RNA Categories: RNA | RNA interference | Biotechnology stubs Views Article Discussion this page History Personal tools Log in / create account Navigation Main page Contents Featured content Current events Random article Search Go Search Interaction Community portal Recent changes Contact Donate to Help Toolbox What links here Related changes Upload file Special pages Printable version Permanent link Cite this page Languages Italiano Svenska 中文 This page was last modified on 16 June 2008, at 23:06
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